Detection of DNA Damage in White Spot Syndrome Virus –Infected Shrimp (Peneaus Mondon) by Using Comet Assay
نویسندگان
چکیده
منابع مشابه
Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay
White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity,...
متن کاملDesign and Evaluation of a PCR Method for Detecting White Spot Syndrome Virus in Shrimp
Background: White spot syndrome virus (wssv) is the causing agent for white spot disease in shrimp and many crustaceans. This disease is highly contagious and can cause death within 3–10 days under normal culture conditions. Therefore, early diagnosis of the virus is a necessity. Materials and Methods: Primers were designed for three regions of the virus genome and one region of the shrimp geno...
متن کاملDiagnosis of endosulfan induced DNA damage in rohu (Labeo rohita, Hamilton) using comet assay
Use of different pesticides in the agriculture sector, in order to boost crop yield within a short time period and low labor, has been tremendously increased since the last decade. Pesticide use has elevated crop yield but has produced a number of pronounced problems regarding environmental and health safety. The continuously deteriorating toxicological effects of these pesticides are not only ...
متن کاملDiagnosis of endosulfan induced DNA damage in rohu (Labeo rohita, Hamilton) using comet assay
Use of different pesticides in the agriculture sector, in order to boost crop yield within a short time period and low labor, has been tremendously increased since the last decade. Pesticide use has elevated crop yield but has produced a number of pronounced problems regarding environmental and health safety. The continuously deteriorating toxicological effects of these pesticides are not only ...
متن کاملA Novel Detection Platform for Shrimp White Spot Syndrome Virus Using an ICP11-Dependent Immunomagnetic Reduction (IMR) Assay
Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 ...
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ژورنال
عنوان ژورنال: Animal and Veterinary Sciences
سال: 2014
ISSN: 2328-5842
DOI: 10.11648/j.avs.20140204.11